Fig. 6

Immune activation mechanism in vivo. A Percentage and statistical analysis of CD3 + CD8 + T cells detected by flow cytometry (n = 5). B PD-L1 expression detected by flow cytometry and western blotting after different treatments (n = 5). C Percentage and statistical analysis of Tim3 + PD-1 + cells detected by flow cytometry (n = 5). D TOX expression detected by flow cytometry and western blotting after different treatments (n = 5). E Cytokines of IFN-γ and granzyme B in CD8 + T cells detected by flow cytometry (n = 5). **P < 0.01, ***P < 0.001, ****P < 0.0001 by one-way analysis of variance (ANOVA) with Turkey’s multiple comparisons