Fig. 2

Structure-guided linker design. a Two trimerization scaffolds used in this study. b The left panel: The trivalent construct designed to match the geometry of the three binding sites on RBDs (orange) of the S protein (silver). The monovalent binder and the trimerization scaffold are shown in blue and green, respectively. The right panel: The calculated minimum distances required between the binder and scaffold. c Schematic of linker models generated by RosettaRemodel. For each model, the distance from the C-terminus of the binder to the N-terminus of the scaffold was determined. d Distributions of the mentioned distances of the designed linkers of different lengths. The dotted black lines indicate the minimum distances required for the constructs