Fig. 1
Hsa_circ_0004085 levels abnormally elevated in tumor tissues and plasma of CRC patients infected with Fn. A FISH was conducted in cohort 1 to identify four CRCicFn+ tissues (left) and four CRCicFn− tissues (right). B, C Differential expression profiles of circRNAs between CRCicFn+ tissues and CRCicFn− tissues were detected by next-generation sequencing, and 70 circRNAs with the most significant differences in expression levels are presented in a heatmap and volcano plot. D, E Expression levels of 6 circRNAs were measured by qRT-PCR in tumor tissues and plasma of 16 CRCicFn+ and 16 CRCicFn− patients in cohort 2. F, G Expression levels of 4 circRNAs in CRC tumor primary cells and CRC cell lines infected with Fn were determined by qRT-PCR. H The circRNAs with abnormally high expression in the icFn+ group were selected by different tests at both the tissue and cellular levels. (*P < 0.05, **P < 0.01, ***P < 0.001, NS: not signifcant)