Fig. 3
Fn infection increased exosomes secretion and promoted the enrichment of hsa_circ_0004085 in exosomes. A Morphologies of ExicFn+, ExicFn−, and ExicEc+ were observed using TEM. B–D The size and number of ExicFn+, ExicFn−, and ExicEc+ cells were analyzed by NTA. E Western blot detected protein levels of exosome-positive biomarkers (CD9 and CD63) and negative markers (β-tubulin) in ExicFn+, ExicFn−, ExicEc+ and CRC cells. F The expression levels of hsa_circ_0004085 in culture medium, exosomes and culture medium without exosomes were determined by qRT-PCR. G The expression levels of hsa_circ_0004085 in ExicFn+, ExicFn−, and ExicEc+ were determined by qRT-PCR. H The levels of hsa_circ_0004085 in CRC cells treated with lentiviral vector were determined by qRT-PCR. I Western blot analysis of the levels of core members in hnRNPs family in different group of LOVO cells. J RIP experiments tested the binding of hnRNPs to pre-EPHB2 or hsa_circ_0004085. K Changes in hsa_circ_0004085 or EPHB2 mRNA levels were determined by qRT-PCR after knockdown or overexpression of hnRNP L. L The expression levels of hsa_circ_0004085 in different group of LOVO cells were determined by qRT-PCR. M The expression levels of hsa_circ_0004085 in exosomes or cells in different group were determined by qRT-PCR. N The expression levels of hsa_circ_0004085 in exosomes from different groups were determined by qRT-PCR. (*P < 0.05, **P < 0.01, ***P < 0.001, NS: not signifcant)