Fig. 4
ExicFn+ transmitted resistance to oxaliplatin/5-FU by delivering hsa_circ_0004085 in vitro. A Tracer analysis of exosomes recorded the uptake of 10 µg PKH26 (red)-stained ExicFn+ by 1 × 105 cells after incubation together for 0 h, 2 h and 6 h. DAPI (blue): cell nucleus, Phalloidin (green): cytoskeleton. B CRC cells were transfected with the Cy3-tagged hsa_circ_0004085 expression vector (orange-red), and exosomes in the medium were isolated and labeled with PKH67 (green). The location of Cy3-hsa_circ_0004085 and PKH67-labeled exosomes was observed after coincubation with recipient CRC cells. C The expression levels of hsa_circ_0004085 in CRC cells incubated with ExicFn+, ExicFn− or ExicEc+ were determined by qRT-PCR. D–F Resistance to Oxa/5-Fu of LOVO cells incubated directly with ExicFn+, ExicFn− or ExicEc+ was analyzed by CCK-8 analysis, colony formation and EdU experiments. G Oxa/5-Fu-induced apoptosis of LOVO cells incubated directly with ExicFn+, ExicFn− or ExicEc+ was tested with flow cytometry. (*P < 0.05, **P < 0.01, ***P < 0.001, NS: not signifcant)