Fig. 7
Hsa_circ_0004085 regulated key molecules in the UPR. A, B Western blot analysis of the levels of UPR-associated proteins in LOVO cells treated with Oxa and 5-FU. C Changes in GRP78 mRNA and ATF6 mRNA levels were determined by qRT-PCR after knockdown or overexpression of hsa_circ_0004085. D The protein level and distribution of GRP78 and ATF6 in CRC tissues with high or low hsa_circ_0004085 expression levels were detected by IHC (serial sections). E The correlation between hsa_circ_0004085 expression and the H-score of GRP78 and nuclear ATF6 (ATF6p50) was analyzed in CRC tumor tissues. F The pull-down products of hsa_circ_0004085 and the control probe were subjected to silver staining. G LC–MS/MS analysis of the pull-down products of hsa_circ_0004085 and the control probe. H The products of RNA pulldown and IB were analyzed by Western blot with anti-RRBP1 antibody. I Hsa_circ_0004085 was measured by qRT-PCR in immunoprecipitates of RRBP1. J Western blotting was used to detect the influence of hsa_circ_0004085 on RRBP1. (*P < 0.05, **P < 0.01, ***P < 0.001, NS: not signifcant)