Fig. 8

Regulatory mechanism of hsa_circ_0004085 to GRP78 and ATF6. A QRT-PCR detected the influence of RRBP1 on GRP78 mRNA. B Western blot analysis of the influence of hsa_circ_0004085 and RRBP1 on GRP78. C QRT-PCR detected the influence of the hsa_circ_0004085 vector and si-RRBP1 on GRP78 mRNA (left). qRT-PCR was used to detect the influence of sh-hsa_circ_0004085 and RRBP1 vectors on GRP78 mRNA (right). D Subcellular localization of hsa_circ_0004085 and RRBP1 was detected by FISH combined with IF. E RIP experiments tested the effect of hsa_circ_0004085 on the binding of RRBP1 to GRP78 mRNA. F IF showed the effect of hsa_circ_0004085 on the nuclear translocation of ATF6p50 in LOVO cells. G Western blot analysis of the effect of hsa_circ_0004085 on the nuclear expression of ATF6p50 in LOVO cells. H The pulldown products of the hsa_circ_0004085 probe and IB products of the anti-ATF6 antibody were analyzed by Western blotting (left). Hsa_circ_0004085 was measured by qRT-PCR in immunoprecipitates of ATF6. (*P < 0.05, **P < 0.01, ***P < 0.001, NS: not signifcant)