Fig. 3
VNP-shCCDC25 inhibits tumor metastasis. A The titer of bacterium colonized in the lung tumor at 4 h post i.v. with 1 × 106 CFU VNP-NC or VNP-shCCDC25 (n = 3). B The treatments schedule for VNP-shCCDC25 inhibition of tumor metastasis in B16F10 lung metastasis model: 3 days after B16F10 cells injection, PBS, VNP-NC, or VNP-shCCDC25 was administrated by i.v. and the tissues were collected at 10 days. C, D The pictures and numbers of the lung metastasis foci in B16F10 lung metastasis model (n = 6). Scale bars: 2000 μm. E The treatment schedule for VNP-shCCDC25 inhibition of tumor metastasis in 4T1 orthotopic lung metastasis model: 18 days after 4T1 cells injection, PBS, VNP-NC, or VNP-shCCDC25 was administrated by i.v. and the tissues were collected at 28 days. F, G The pictures and numbers of the lung metastasis foci in 4T1 orthotopic lung metastasis model. Scale bars: 2000 μm. H H&E staining of the lung metastasis foci after treatments in 4T1 orthotopic lung metastasis model. Scale bars: 1000 μm. I H&E staining of situ tumors after treatments in 4T1 orthotopic lung metastasis model. Scale bar: 200 µm. N: necrotic region, nN: nonnecrotic region. Data are shown as the mean ± SD. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns: no significance