Fig. 5
VNP-shCCDC25 blocks the downstream pro-metastasis signaling pathway of CCDC25 and reduces the formation of NETs. A 16 h after co-incubation with VNP-shCCDC25 and B16F10 cells (MOI = 10:1), the expression level of genes in the downstream prometastasis signaling pathway of CCDC25 in B16F10 cells was analyzed via qPCR. B After VNP-shCCDC25 treatment, the expression level of CCDC25 in the metastatic foci of B16F10 lung metastasis model was analyzed via qPCR. C After VNP-shCCDC25 treatment, the expression level of PAD4 in the metastatic foci of B16F10 lung metastasis model was analyzed via qPCR. D In 4T1 orthotopic lung metastasis model, the expression level of genes in the downstream prometastasis signaling pathway of CCDC25 in the lung tissue after administrations was analyzed via qPCR. E, F The number of MPO in metastatic foci of B16F10 lung metastasis model was analyzed via immunohistochemistry (IHC). G IHC analysis of MPO expression in metastatic foci of 4T1 orthotopic lung metastasis model. Data are shown as the mean ± SD. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns: no significance