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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: M2 macrophage-derived exosomal miR-26b-5p regulates macrophage polarization and chondrocyte hypertrophy by targeting TLR3 and COL10A1 to alleviate osteoarthritis

Fig. 3

miR-26b-5p regulates TLR3 signaling pathway. A pmirGLO plasmids were constructed with WT or Mut 3’ UTR binding site sequences of TLR3. B Dual luciferase reporter assay showed the inhibition of miR-26b-5p on TLR3 expression in HEK293T cells. The expression of TLR3 after M1-CM stimulation was assessed by immunofluorescence staining (C) and western blotting (D). E Effects of miR-26b-5p overexpression on TLR3 signaling pathway. *p < 0.05, **p < 0.01

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