Fig. 5

Inhibitory effect of miR-26b-5p on chondrocyte hypertrophy. A pmirGLO plasmids were constructed with WT or Mut 3′ UTR binding site sequences of COL10A1. B Inhibitory effect of miR-26b-5p on COL10A1 expression was assessed via a dual luciferase reporter assay. C Expression levels of RUNX2, COL10A1, COL1A1, COL2A1, SOX9, and Aggrecan in ADTC5 cells treated with M1-CM were examined via qRT-PCR. D ADTC5 cells were stimulated with M1-CM during chondrogenesis induction and stained with Alizarin red. E Western blotting analysis showed the protein expression of RUNX2, COL10, COL1, COL2, and SOX9. *p < 0.05, **p < 0.01, NS not significant