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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Multifunctional biomimetic hydrogel dressing provides anti-infection treatment and improves immunotherapy by reprogramming the infection-related wound microenvironment

Fig. 4

Macrophage behavior on various PADM-based hydrogels. (a) After different hydrogel treatments, macrophages were detected for the expression of M1 (iNOS: green) and M2 (CD206: red) marker proteins using CLSM. Scale bar: 20 μm. (b) 3D surface plot of iNOS (green) and CD206 (red). (c) Statistical analysis of iNOS fluorescence intensity. (d) Statistical analysis of CD206 fluorescence intensity. (e) The surface markers CD86 and CD206 of RAW 264.7 after different hydrogel treatments were analysed by flow cytometry. (f) Positive CD86 and CD206 (g) cell percentage. Results are presented as mean ± SD (N = 3). ***P < 0.001; ****P < 0.0001

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