Fig. 1

Identification of the exosome from hADSC and hUCMSC. a Morphology of hADSC and hUCMSC. Scale bar = 100 μm. b Multiple differentiation potential of hADSC and hUCMSC. Scale bar = 25 or 100 μm. c Surface markers profiling of hADSC and hUCMSC. The cells were highly positive for CD105, CD90, and CD73 and were negative for HLA-DR and CD45. d By TEM, purified hADSC-Exo and hUCMSC-Exo exhibit cup-like morphologies. e Nanoparticle analysis of hADSC-Exo and hUCMSC-Exo. f The protein markers in hADSC, hUCMSC, and their exosomes were analyzed by Western blot. hADSC-Exo and hUCMSC-Exo can express their common positive markers CD81, CD63, and CD9, however, the negative marker Calnexin is not expressed. WB analysis of whole cell lysates of the hADSCs and hUCMSCs shown in f