Fig. 5

BTBR mice brains treated with hADSC-Exos showed reduced inflammation and increased neurogenesis in the PFC region. a–d Immunofluorescence double-staining of formalin-fixed mice brain sections. a Staining for committed neuronal markers GFAP (green immunofluorescence) and Tuj1 staining (red immunofluorescence) in the PFC is shown, and DAPI staining (blue fluorescence). b Iba-1 fluorescence in green and Tuj1 immunofluorescence in red (Texas Red), and DAPI staining (blue fluorescence). c Representative immunofluorescence images reveal Tuj1 (green) and CC-3 (red) immunoreactive (Tuj1 + /CC-3+) cells in the PFC region. d The red color is vGlut1 staining and the green color is GAD67, and nuclear staining by DAPI staining (blue fluorescence). A two-way ANOVA followed by Tukeys multiple comparison test was applied to all data. Error bars represent S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001