Fig. 2

RmEVs improve intestinal development in vivo during early life (n = 7). (A) Animal designs and mEVs administration schedule in young C57BL/6 mice. (B) The body weight of mice. the spherical test results were not satisfied (p = 0.01), the difference between groups was significant (p = 0.03), and interaction is not existed (time*group = 0.08). The body weight at each time point was analyzed separately and the superscript means the difference degree between RmEVs and CmEVs/Blank. (C) The length of the whole intestine of mice. (D) The images of HE dye in the jejunum, scale bar: 200 μm. (E) The crypt depth of jejunum. (F) The villi length of jejunum. (G, H) EdU positive cells observation and assay in the jejunum of mice. Sections were stained with EdU and DAPI, scale bar: 100 μm. The quantitative analysis of EdU positive cells by Image J. (I) The positive CCND-1 protein in the jejunum, scale bars: 50 μm. (J, K) The relative mRNA expression of IGF-1R and PCNA in the jejunum. Continuous data of body weight were expressed as mean ± SD, others are expressed as means ± SEM. *p < 0.05, **p < 0.01; ns, not significant