Fig. 3

RmEVs promote cell proliferation and migration of IEPC-J2 (n = 3). (A-C) The assays of the proliferation of IPEC-J2 by CCK8 assay (A), MTT assay (B), and EdU assay (C). (D-F) Wound-healing assay. IPEC-J2 cells were treated with RmEVs, CmEVs, and Blank. Images were taken on the 0, 12, and 24 h after the scratch, scale bar: 400 μm. The spherical test results were not satisfied (p = 0.02), the difference between groups was significant (p = 0.04), and interaction did not exist (time*group = 0.22). The percentage of scratched area recovery of IPEC-J2 at 12 h (E) and 24 h (F) were analyzed separately. (G-I) The relative mRNA expression of CDX2, PCNA, and CCND-1. (J) Images of PKH26-labeled mEVs absorbed by IPEC-J2 after 6 h of culturing, scale bars = 50 μm. (K) The PKH-26 positive cell rates of IPEC-J2. Continuous data of recovery rate were expressed as mean ± SD, others are expressed as means ± SEM. *p < 0.05, **p < 0.01; ns, not significant