Fig. 4

miR-146a-5p increases the proliferation and migration of IPEC-J2 (n = 3). (A) The images of EdU, scale bar: 50 μm. (B) The percentages of EdU in IPEC-J2. (C) Images of scratched recovery of IPEC-J2 cells after being transfected by miR-146a-5p mimics, inhibitors, mimics-NC, inhibitors-NC, scale bar: 400 μm. (D, E) The percentage of scratched area recovery of IPEC-J2. Data were analyzed by repeated measures analysis of variance, followed by within-group comparisons. The spherical test results were not satisfied (p = 0.04), the difference between groups was significant (p = 0.001), and interaction did not exist (time*group = 0.86). The scratched area recovery of IPEC-J2 at 12 h (D) and 24 h (E) were analyzed separately. (F, G) The cell proliferation of IPEC-J2 treated with RmEVs + inhibitors-NC, RmEVs + miR-146a-5p inhibitors, or Inhibitors-NC for 24 h followed by CCK (F) and MTT assays (G). Continuous data of recovery rate were expressed as mean ± SD, others are expressed as means ± SEM. *p < 0.05, **p < 0.01