Fig. 5

miR-146a-5p enhances the activation of Wnt pathway via targeting NEDD4L (n = 3). (A) The potential binding sites of miR-146a-5p in NEDD4L 3’UTR and their wild (WT) or mutant (MUT) sequence designs. (B) Luciferase reporter assays. HEK239 cells were co-transfected with pirGLO vector-WT/MUT and miR-146a-5p mimics or negative control. WT, wild type; MUT, mutant type; NC, negative control. (C-I) The relative mRNA expression of NEDD4L, DVL2, AXIN2, c-MYC, GSK-3β, β-catenin, and CCND-1 in IPEC-J2 cells after transfected with miR-146a-5p. (J-M) The protein levels of NEDD4L, DVL2, and β-catenin after transfected with miR-146a-5p. Data are expressed as means ± SEM. *p < 0.05, **p < 0.01; ns, not significant