Fig. 7

Overexpression of miR-146a-5p improves renewal of intestinal cells in young mice models (n = 8). (A) Animal designs and schedule. (B) The changes in body weight during the experiment. The spherical test results were satisfied (p = 0.144), the difference between groups was significant (p = 0.01), and interaction did not exist (time*group = 0.06). The body weight at each time point was analyzed separately and the superscript means the difference degree between agomiR-146a-5p and NC/Control. (C) The representative images of the intestine of mice from different treatments. (D-G) Quantitative analysis of intestinal length and intestinal weight of mice from different treatments. (H) Images of Cy3 and DAPI fluorescence in the jejunum of mice at 6 h after the injection of Cy3-labeled agomiR-NC, Cy3-labeled agomiR-146a-5p, or PBS. Scale bar: 50 μm. (I) The expression of miR-146a-5p in the jejunum. (J, K) Images of NEDD4L protein expression in the jejunum, presented by IHC method and quantitative analysis, scale bar: 50 μm. Continuous data of body weight were expressed as mean ± SD, others are expressed as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant