Fig. 8
Mechanism of NVRBD-MLipo in eliciting immune responses. Lung was harvested from mice at 24 h post immunization and RNA was further isolated for transcriptomic analysis. (A) Schematic for transcriptomic analysis of lung. (B) Principal component analysis (PCA) of lungs treated with different groups (n = 3). Volcano plots (C) and heat map (D) for differentially expressed genes (DEGs) (n = 3). (E) KEGG enrichment analysis of DEGs (n = 3). (F) FCM analysis of TLR4 activation in RAW 264.7 cells following treatment with RBD and NVRBD-MLipo for 12 h. (G) Western blot analysis of NF-κB and p-NF-κB expression in RAW 264.7 cells. Cells were treated with different groups as described in (F)