Fig. 4

Nanoparticle localization in Min deficient cells. A, D Representative TEM micrographs of ΔminC and ΔminCDE strains after biosynthesis conditions. Red arrows show spots with electron-dense nanoparticles inside the cell. B, E Digital zoom of images (A) and (D), respectively, highlighting minicells in formation. C, F Size histograms of nanoparticles inside minicells of (B) and (E), respectively. G EDS analysis of a minicell from ΔminC strain after biosynthesis conditions