Fig. 5

Minicell enrichment. A Diagram of the separation process of minicells used in this work. Full description of the process is included in “Materials and Methods”. B Cell pellets of mutant strains exposed to UV light (365 nm) for fluorescence detection. Fractions of the whole strain (previous to separation), minicells and rod cells are shown. C Representative microscopy images of enriched fractions of minicells and rod cells. Fluorescence microscopy images were captured after excitation with a 330–380 nm filter