Table 1 Primers used in this study
Name | Sequence (5’–3’) | Construction/purpose |
|---|---|---|
minC-P1 | GCAGAACCTAAGGTTATCCATCAGGCGCTGGAAGACAAAATCGCTGTGTAGGCTGGAGCTGCTTC | ΔminC::Cm and ΔminCDE::Cm |
minC-P2 | CGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGAATGGGAATTAGCCATGGTCC | ΔminC::Cm |
minCDE-P2 | AAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACATGGGAATTAGCCATGGTCC | ΔminCDE::Cm |