Fig. 1
From: Spike structure of gold nanobranches induces hepatotoxicity in mouse hepatocyte organoid models
Generation of mice Hep-orgs with hepatocyte characteristics. (a) Bright-field optical images of organoids in EM and DM. (b) CLSM images of Hep-orgs stained with F-actin and DAPI, showing a single plane in a 3D scan. (c) Relative gene expressions of EPCAM and ductal markers (Sox9, CFTR) in organoids cultured in EM and DM, with GAPDH as the internal control. (d) Relative gene expressions of hepatocyte markers (AFP, HNF4α, ALB, CYP3α11, Ttr) in organoids cultured in EM and DM, with GAPDH as the internal control. (e) CLSM images showing the expressions of liver markers CK19, HNF4α, Desmin, E-Cad, and ZO-1 in Hep-orgs. All data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001