Fig. 5
From: Spike structure of gold nanobranches induces hepatotoxicity in mouse hepatocyte organoid models
The effects of GNSs/GNBs on the structural and physiological states of Hep-orgs. (a) CLSM 3D composite images of Hep-orgs cell cytoskeleton after treatment with GNSs and GNBs. The scanning height was 200 μm with a scale interval of 5 μm. (Red: F-actin; Blue: DAPI) (b) Corresponding fluorescence intensity ratio of nuclear/F-actin in Hep-orgs. (c) CLSM 3D composite images of MMP changes in Hep-orgs after the treatment with GNSs and GNBs. The scanning height was 300 μm with a scale interval of 5 μm. (Red: J-aggregates; Green: Monomer) (d) Ratio of JC-1 green/red fluorescence intensity in Hep-orgs. (e) CLSM 3D composite images of ROS production in Hep-orgs after the treatment with GNSs and GNBs. The scanning height was 250 μm with a scale interval of 5 μm. (f) Corresponding fluorescence intensity of ROS production in Hep-orgs. All data are presented as mean ± SD (the dots represent the number of samples). *P < 0.05, **P < 0.01, ***P < 0.001