Table 1 Real-Time PCR Primers and Conditions
From: Spike structure of gold nanobranches induces hepatotoxicity in mouse hepatocyte organoid models
Gene | Genbank Accession | Primer Sequences(5’to3’) | Size(bp) | Annealing (℃) |
|---|---|---|---|---|
Mouse GAPDH | GU214026.1 | GAAGGTCGGTGTGAACGGATTTG | 127 | 60 |
Mouse AFP | NM_007423.4 | GCTCACATCCACGAGGAGTGTT | 153 | 60 |
| Â | Â | GCAGAAGCCTAGTTGGATCATGG | Â | Â |
Mouse ALB | NM_009654.4 | CAGTGTTGTGCAGAGGCTGACA | 107 | 60 |
| Â | Â | CTGGAGCACTTCATTCTCTGACG | Â | Â |
Mouse CFTR | NM_021050.2 | CTTCGCTGGTTGCACAGTCA | 105 | 60 |
| Â | Â | GAGTCGTACTGCCAGACATTG | Â | Â |
Mouse CYP3A11 | NM_007818.3 | CAGCACTGGTCAGAGCCTGAA | 132 | 60 |
| Â | Â | GAGAGCAAACCTCATGCCAAGG | Â | Â |
Mouse EPCAM | NM_008532.2 | GTCATTGTGGTGGTGTCATTAGC | 106 | 60 |
| Â | Â | CCATCTCCTTTATCTCAGCCTTCT | Â | Â |
Mouse HNF4A | NM_008261.3 | GCGAACTCCTTCTGGATGACC | 104 | 60 |
| Â | Â | CAGCACGTCCTTAAACACCATGG | Â | Â |
Mouse Krt19 | NM_008471.3 | GGCGAGCTGGAGGTGAAGA | 132 | 60 |
| Â | Â | GGAGTTGTCAATGGTGGCACCAA | Â | Â |
Mouse SOX9 | NM_011448.4 | ACCCACCACTCCCAAAACC | 114 | 60 |
| Â | Â | ATGTCCACGTCGCGGAAGT | Â | Â |