Fig. 3

Cell uptake and targeting capability of YSA-SPION-MV in vitro. Flow cytometry analysis and bar graph of PKH67 positive cells: i) incubation with MV or YSA-SPION-MV for 6 h (A, B); ii) incubation with YSA-SPION-MV for 6 h or 12 h (C, D). E Fluorescence images of PKH67-labeled YSA-SPION-MV treated 143B and MG63 cells with and without blocking by YSA peptide, and SK-MEL-28 cells (scale bars: 50 μm). *P < 0.05; **P < 0.01; ***P < 0.001