Fig. 5

In vitro cytotoxicity of YSA-SPION-MV/MTX. A Bright field images of morphology of tumor cells after treatment with PBS (Control), YSA-SPION-MV, MTX, MV/MTX and YSA-SPION-MV/MTX for 24 h. The dose was equivalent to 5 μg/mL MTX (scale bars: 50 μm). B, C Flow cytometry analysis based on Annexin V-FITC/PI staining of tumor cells after corresponding treatment for 24 h. D Relative cell viability of tumor cells was detected by CCK-8 assay after corresponding treatment for 24 h. Ns: no significance, *P < 0.05; **P < 0.01; ***P < 0.001