Fig. 7

In vivo anti-tumor effect of YSA-SPION-MV/MTX. A Bioluminescent images and B luminescence intensity quantification of tumor-bearing mice in control, YSA-SPION-MV, MTX, MV/MTX and YSA-SPION-MV/MTX groups (the dose was equivalent to 5 mg/kg MTX). C General view of the right hindlimbs from tumor-bearing mice receiving corresponding treatment for 28 days. D Tumor weights were measured after corresponding treatment. E Tumor volume growth curves of tumor-bearing mice during the administration of corresponding treatment. F H&E staining, immunohistochemistry analysis (Cleaved Caspase-3) and TUNEL analysis of tumor in different groups. Scale bars: 50 μm. Ns: no significance, *P < 0.05; **P < 0.01