Fig. 8

Ex vivo PA-US imaging of melanin-coated AuNPs and AuNS. PA-US images, PA spectra and PA intensities at 710 nm of melanin-coated (a, b, c) AuNPs, (d, e, f) AuNS HEPES, (g, h, i) AuNS EPPS and (j, k, l) AuNS MOPS intramuscularly injected (50 µL, 0 or 200 µM gold) in the legs of deceased mice. The color in the PA-US images represent the PA intensity at 710 nm. (**) and (***) indicate groups that are significantly different with p < 0.01 and p < 0.001, respectively (independent two-sample t-test). Values in columns represent mean ± standard deviation of three different batches of nanoconstructs. Yellow dashed areas in (a, d, g, j) indicate the regions of interest used for the spectral acquisition