Fig. 7
The in vitro bioactive effect of the NO gas on the proliferation, adhesion and angiogenic performance of HUVECs. a Images of the tube formation experiment with HUVECs in the control, SCMC@MNs, SNP-Fe@MNs-NO(−),and SNP-Fe@MNs-NO(+) groups. b The relative data is expressed as the mean ± S.D. from three independent experiments. c, d Scratch images of HUVECs migration after coculture with SCMC@MNs, SNP-Fe@MNs-NO(−), and SNP-Fe@MNs-NO(+) groups and relative migration ratio. e OD 450 values of HUVECs after being treated by MNs for 1 and 2 days. f–h Angiogenesis-related gene expression of VEGF, HIF-1a, and eNOs in HUVECs. i–n Protein levels of HIF-1a, VEGF, AKT, p-AKT, and ratio of p-AKT/AKT after treated by MNs were detected by Western blot (n = 6. *P < 0.05; **P < 0.01; ***P < 0.0001)