Fig. 4
The mechanism of HA/H-I NPs inducing tumor cell apoptosis. (A) Fluorescence microscopy images analysis for intracellular ROS generation of RKO cells using DCFH-DA as a probe. Scale bar: 50 μm. (B) Flow cytometry analysis for intracellular ROS generation of RKO cells using DCFH-DA as a probe. (C) Fluorescence microscopy images analysis for intracellular ROS generation of HCT116 cells using DCFH-DA as a probe. Scale bar: 50 μm. (D) Flow cytometry analysis for intracellular ROS generation of HCT116 cells using DCFH-DA as a probe. (E) Immunoblot analysis of autophagy markers for RKO cells treated with different treatments. (F) Immunoblot analysis of apoptotic markers for HCT116 cells treated with different treatments. (G) Immunoblot analysis of autophagy markers for RKO cells treated with different treatments. (H) Immunoblot analysis of apoptotic markers for RKO cells treated with different treatments