Fig. 5
miR-9-5p was the candidate effector of iPSC-MSC-EXOs in attenuation of DOX-induced cardiomyocyte senescence. A RNA-seq analysis revealed the differential expression of miRNAs between BM-MSC-EXOs and iPSC-MSC-EXOs. B The expression level of miR-9-5p in iPSC-MSC-EXOs was validated by qPCR. C The expression level of miR-9-5p in iPSC-MSCs and miR-9-5pKD- iPSC-MSCs was validated by qPCR. D The expression level of miR-9-5p in iPSC-MSC-EXOs and miR-9-5pKD-iPSC-MSC-EXOs was validated by qPCR. E, F Representative images and quantitative measurement of mitochondrial fragmentation in NMCM treated with iPSC-MSC-EXOs or miR-9-5pKD-iPSC-MSC-EXOs under DOX challenge. G, H Representative images and quantitative measurement of SA-β-gal positive cells in NMCM treated with iPSC-MSC-EXOs or miR-9-5pKD-iPSC-MSC-EXOs under DOX challenge. n = 3 biological replicates for each group. Data are expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001