Fig. 5

Effect of miR-100-5p on inflammation and proliferation of RA-FLS. (A) mRNA expression of miR-100-5p in RA-FLS transfected with NC mimics or miR-100-5p mimics analyzed by qRT-PCR. (B) Images of RA-FLS transfected with NC mimics-cy3 or miR-100-5p mimics-cy3. (C) Proliferation of RA-FLS transfected with NC or miR-100-5p mimics and stimulated with or without 10 ng/mL IL-1β or 25 ng/mL TNF-α at 24, 48, 72, and 96 h by CCK8. (D) mRNA expression of IL-1β, IL-6, IL-8, MMP-1, and MMP-13 in RA-FLS transfected with NC or miR-100-5p mimics and stimulated with or without 25 ng/mL TNF-α. (E) mRNA expression of TNF-α, IL-6, IL-8, MMP-1, and MMP-13 in RA-FLS transfected with NC or miR-100-5p mimics and stimulated with or without 10 ng/mL IL-1β. Data are presented as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001