Fig. 5

Synergistic antitumour effects of Hm@TSA/As-MOF and α-PD-1 in orthotopic mouse models of HCC. Mice with HCC were subjected to the treatment regimen illustrated in panel (A). Tumours were assessed via in vivo bioluminescence imaging (B). Representative images of tumours from each group of mice are shown (C). Tumour weight (D), tumour volume (E), tumour index (F) and TSR (G) were quantitatively analysed (n = 5). The histological structure and proliferative capacity of tumour tissues were evaluated via HE (H), Ki67 (I) and TUNEL (J) staining (scale bar = 100 μm). Tumour cell apoptosis was assessed via flow cytometry (K). The survival of mice in each group was monitored daily (L) (n = 8). Data are expressed as the mean ± SD (*, p < 0.05; **, p < 0.01; ***, p < 0.001 based on two-tailed Student’s t-test)