Fig. 2
T cell-targeting capability and biodistribution of MVMαCD3chDC. A Intracellular uptake of MVMchDC and MVMαCD3chDC in T cells after 8 h incubation. MVMchDC and MVMαCD3chDC were labeled using DiD (red). T cell membrane was stained with FITC secondary antibody (green). Scale bar = 5 μm. B The corresponding intracellular fluorescence signals were determined by flow cytometry analysis. CEx vivo tissue distribution of MVMchDC and MVMαCD3chDC. H : heart; Li : liver; S : spleen; Lu : lung; K : kidney. Mice were intravenously injected with MVMchDC and MVMαCD3chDC at different time points. MVMchDC and MVMαCD3chDC were labeled using DiD fluorescence. D Quantitative analysis of MVMchDC and MVMαCD3chDC in heart, liver, spleen, lung, and kidney at different time points. E The average radiance ratio of spleen to liver for MVMchDC and MVMαCD3chDC at different time points. F Uptake of DiD labeled MVMchDC and MVMαCD3chDC by myeloid and lymphoid cells in spleen at 24 h. Data were presented as mean ± SD. Statistical analyses were performed by Student’s t-test. N = 3 per group. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001