Fig. 4
MVMchDC promoted the proportion of Treg through upregulated miR-155-3p. A Differentially expressed microRNA haipin (mir) and mature microRNA (miR) between MVMimDC and MVMchDC were exhibited via a heat map. B Enriched terms of biological process of the upregulated genes by GO analysis. C Enriched terms of KEGG pathway analysis of the up-regulated genes. D-E Pie chart and volcano plot displaying the distribution of the down-regulated, up-regulated, and common miR or mir between MVMimDC and MVMchDC. The red and green dots indicated the differentially expressed miRNAs. F Relative expression of miR-155-3p in imDC and chDC. G Expression levels of β-actin and SOCS1 protein in splenic lymphocytes after different treatments. H Percentage of the CD3+CD4+FoxP3+ Treg in CD3+CD4+ T cells after different treatments using flow cytometry analysis. Data were presented as mean ± SD. Statistical analyses were performed by Student’s t-test or One-way ANOVA with Tukey method. N = 3 per group. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001