Fig. 6

The effect of MVM^αCD3_chDC on joint injury and inflammatory response in the CIA model. A Timeline of MVM^αCD3_chDC treatment experiment in CIA model. B Macroscopic images of hind paws in mice after different treatments. C The change of hind paw thickness and arthritis scores in mice after different treatments. D H&E, SO-FG, and immunohistochemical staining images of histological sections in joints after different treatments. Scale bar = 100 μm. Black arrows indicated bone destruction and narrow joint space. Red arrows indicated invasion of inflammation. Blue arrows indicated loss of proteoglycan. E-F Percentage of the CD3⁺CD4⁺FoxP3⁺ Treg in CD3⁺CD4⁺ T cells in spleen and lymph nodes in CIA mice after different treatments using flow cytometry analysis. G The concentration of TNF-α, IL-6, and IL-10 in the serum of mice after different treatments. Data were presented as mean ± SD. Statistical analyses were performed by One-way ANOVA with Tukey method. N = 6 per group. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001