Fig. 4

A The schematic diagram, the microstructure (SEM) of PCL fiber template, template-cell-ECM, and ECM-C during the preparation process (a). Inset: SEM examination of the transverse sections. RSC96 cells were stained with fluorescein isothiocyanate conjugated phalloidin and DAPI (b). Migration traces of RSC96 cells on different scaffolds (c). Typical images of RSC96 cell and the nuclear shape index of RSC96 cell on different scaffolds (d). Macrophages were detected by co-immunofluorescence staining for CD206 (green, M2)/CD68 (red, M0), and iNOS (green, M1)/CD68 (red, M0) (e). The number of myelinated fibers (f) and Axon diameter (g) among various scaffolds. Reprinted with permission from ref [94]. B Schematic illustration of the fabrication process of pDNM-G scaffolds and SEM imaging of R-pDNM-G and A-pDNM-G scaffold (a). Fluorescence micrographs of nerve cells on A-pDNM-G-L, A-pDNM-G-M, A-pDNM-G-S, and R-pDNM-G scaffolds, respectively (b). Quantitative analysis of the diameter of nerve fibers (c), the number of myelinated axons (d), and diameter of the myelinated axons (e). ^ap < 0.05 between Autograft and the other groups. Reprinted with permission from ref [95]