Fig. 5

A Schematic diagram of fabrication and SEM images of neural stem cells cultured on anisotropic micropillar scaffolds with a distal distance of 6 µm guide (up) and 12 µm guide (down) neurite growth. Scale = 4 µm. Phase contrast figures show the nerve cell growth morphology at different groups (b). Scale = 20 µm. Pie charts show the percentage of neurite branches on glass substrates and micropillar scaffolds with spacing of 3, 6, and 9 µm (c). The orientation of neurites is represented by the orientation angle distribution (d). Reprinted with permission from ref. [101]. B Schematic view of scaffold construction (a). SEM images of the scaffolds stretched at different angles and scheme of tailored inverse films with different stretching directions (b). SEM imaging of cell culture on films stretched in different directions based on angles of 0°-, 15°-, 30°- and 45°-stretched (b) and angle distribution of neurites of PC12 cells (c). Reprinted with permission from ref [109]