Fig. 3

CDT with TCuH NPs in an H₂O₂/GSH environment and photothermal performances of TCuH after incubation with various concentrations of NaHS. (A) Schematic diagram of TCuH and NaHS reacted to generate photothermal agent Cu₉S₈ and TCuH reduction to Cu⁺ by excess GSH, which catalyzed production of •OH from excess H₂O₂. (B) Electron spin resonance spectra of TCuH NPs (10 µg/m) with < 10 mmol GSH/H₂O₂. (C) Time-dependent UV-Vis absorption spectra of MB-containing TCuH NPs (10 µg/mL) in solution without GSH/H₂O₂. (D) Time-dependent UV-Vis absorption spectra of MB-containing TCuH NPs (10 µg/mL) in solution with GSH (10 mM) and without H₂O₂. (E) Time-dependent UV-Vis absorption spectra of MB-containing TCuH NPs (10 µg/mL) in solution with GSH/H₂O₂ (10 mmol). (F) Normalized absorbance of the MB solution in the presence of TCuH NPs (10 µg/mL) with/without the addition of H₂O₂ and GSH. (G) HR-XPS of Cu 2p (Cu₉S₈). (H) HR-XPS of S 2p (Cu₉S₈). (I-M) Representative temperature evolution curves of aqueous dispersions of TCuH NPs at various concentrations (25, 75, and 100 µg/mL) in the presence of NaHS under 1064 nm laser irradiation at 1.0 W/cm². (N) Corresponding thermal images of TCuH NPs (100 µg/mL) in the presence of NaHS (3 mmol) under 1064 nm laser irradiation for various times (0–7 min)