Fig. 4.

18S rRNA:DNA rectangles stability assays. a Shelf life, thermal stability of 18S rRNA-DNA rectangles at 37 °C (blue) and 50 °C (yellow) over a month. b DNase I assay. Stability profile of bare (red) and K₁₀-PEG_5K coated (blue) 18S rRNA-DNA rectangles at 37 °C in the presence of DNase I over 24 h. c RNase H assay. Stability profile of bare (red) and K₁₀-PEG_5K coated (blue) 18S rRNA-DNA rectangles at 37 °C in the presence of 1.25 units of RNase H over a period of 4 h. d Low Mg⁺² concentration. Stability profile of bare (red) and K₁₀-PEG_5K coated (blue) 18S rRNA-DNA rectangles at 37 °C in different Mg⁺² concentrations over 1 h. e Human serum assay. Stability profile of bare (red) and K₁₀-PEG_5K coated (blue) 18S rRNA-DNA rectangles at 37 °C in human serum over 72 h. (*) represents normalized amount of the folded 18S rectangles to time 0