Figure 2

Full length engineered nucleotide sequence (466 b.p.) coding for the SAPV protein vector. Oligonucleotide primer sequences used to amplify the SAPV DNA are underlined (T7-F forward and T7TER-R reverse primers). The reverse oligonucleotide primer SA-7R was used to amplify SAPV lacking stop codons (to facilitate self-assembly by slowing down transcription and translation). Turquoise highlighting denotes T7 RNA polymerase binding sites, red highlighting – a ribosome binding site, preceding the ATG start codon (light green). Sequence fragment within the SA-7R oligonucleotide highlighted in yellow codes for the amino acid loop within the Streptavidin sequence, which is suitable for modifications (see also Figures 6 and 7). Stop codons are highlighted in blue, the transcription termination site in pink.