Figure 8
BCMP84 immunoprecipitation on protein A-conjugated glass beads and on nitrocellulose membrane. Recombinant BCMP84 was incubated with beads or nitrocellulose that had BCMP84 antibody bound to them. Samples from the first wash, 4th wash and the eluate from these incubations were run as indicated. The washes and eluates from the beads are on the left and the washes and eluates from the filter paper are on the right. White asterisks denote immunoprecipitated and eluted BCMP84 protein, which is not detected in the last (4th) wash prior to elution (both blots). The 1st wash, as expected, includes recombinant BCMP84 as indicated by the band at approximately 40 kDa. This band is not present in the 4th wash. Comparable amounts of the BCMP84 protein are present in the eluate of both the beads and nitrocellulose (marked with asterisks), indicating that it was selectively retained.