Figure 3From: Construction of an artificial cell membrane anchor using DARC as a fitting for artificial extracellular functionalities of eukaryotic cellsWestern Blot of DARC in transfected cells and erythrocytes. In 3a of total membrane fraction of CHO-K1 cells (lane 1) and solubilized membrane fraction of erythrocytes (lane 2) co-incubated with antibodies against the DARC- and the Transferrin receptor. Lane 1 shows the expected band of Transferrin of around 100 kDa. In figure 3b different protein loads are shown: lane 3 carries 10 μg of solubilized erythrocyte membrane; lane 4 = 5 μg; lane 6 = 15 μg; lane 5 = molecular weight marker.Back to article page