Figure 10
From: Microarray-based method for screening of immunogenic proteins from bacteria
Western Blot of whole cell lysates. Four bands are visible in the KRX whole cell lysate with a more prominent band at 70 kDa and three weaker bands at 55 kDa, 28 kDa and roughly 18 kDa. These bands are most likely due to cross-reactive proteins within the E.coli lysates. All four proteins previously described as non-immunogenic (argC, gapA, pseB and pyrC) show band patterns highly similar to KRX. This underlines their lack of immunogenic potential. The five proteins previously described as immunogenic elsewhere show stronger bands at roughly 70 kDa for cjaA, hisJ, peb1a, flaC and 55 kDa for pal respectively. These sizes correspond closely to the expected sizes of the HaloTag® fusion proteins. However, the interference of the background bands from KRX - being in the same range as the target proteins under investigations - prevents a secure identification of immunogenic proteins.