Figure 2

Antibody production in mice immunized with NP+DENV. A) Swiss mice were vaccinated according to the indicated schedule and the sera were collected 1 week after the last immunization. Data presented are values for individual mice and the bar represents the group mean. P < 0.001: PBS versus NP+DENV; DENV versus NP+DENV and NP versus NP+DENV; P < 0.01: PBS versus DENV and DENV versus NP by one-way analysis of variance (ANOVA) followed by Tukey's multiple comparison test. B) Kinetics of IgG production in mice immunized with NP+DENV. The serum were collected on day 0, 7, 14 and 21 post-immunization. Data presented are values for individual mice and the bar represents the group mean. P < 0.01: 0 versus 21 and 7 versus 21 by one-way analysis of variance (ANOVA) followed by Tukey's multiple comparison test.