Figure 6From: Incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cellsThe functionalized nanofiber substratum potentiates cardiomyocyte differentiation. Human PSCs were cultured over the MEF. To induce cardiomyocyte differentiation, EBs were formed by culturing the PSCs over ultra-low-attachment culture dishes. The same number of EBs were spread and differentiated for 10 d over each of the substratum as depicted in the diagram (a). Laminin-211 indicates laminin-211-coated dishes, PEG-AuNP indicates nanofiber substrata containing the PEG-AuNPs, and PEG-AuNP-HBP + cRGD indicates nanofiber substrata containing PEG-AuNPs functionalized with HBP and cRGD. After 10 d of differentiation, cell clumps showing spontaneous contractility appeared. A single cardiomyocyte colony was defined as a cell clump showing synchronized beating (black outlined), irrespective of its size (b). Cardiomyocyte colonies generated in 3 dishes of each substratum were counted for quantification (c). Scale bars: 500 μm. Error bars: SEM, n = 3. The asterisks indicate statistical significance (P < 0.01) calculated using the Student t test. The results shown represent 3 independent experiments. PSC: pluripotent stem cell, MEF: mouse embryonic fibroblast, EB: embryoid body, Growth: growth medium, and Differentiation: differentiation medium.Back to article page