Figure 3
From: Immunoliposome-PCR: a generic ultrasensitive quantitative antigen detection system
Effect of different reagents on the background of the ILPCR assay. Various combinations of the assay reagents were assessed for their effect on the non-specific background signal (noise) of the ILPCR assay. Column A: all assay reagents minus the liposome detection reagent. Column B: all assay reagents minus the antigen, the biotin-labeled secondary antibody, and the NeutrAvidin. Column C: all assay reagents minus the secondary antibody. Column D: all assay reagents minus the antigen, which represents the true assay blank. Column E: all assay reagents minus the antigen (as in D), but with no DNase I digestion step. Measurements were performed using a Bio-Rad model CFX96 real-time PCR system (Hercules, CA).