Figure 4

Inhibited uptake of ricinB:QDs upon cholesterol depletion. The HeLa cells were left untreated (A) or were pretreated with methyl-β-cyclodextrin (mβCD, 10 μM) for 30 min (B and C). Then, the cells were allowed to bind and endocytose ricinB, ricinB:QDs (red), or Tf-alexa647 (green) for 30 min. The cells were fixed and stained with an antibody against EEA1 followed by the appropriate secondary ab-Cy2 (green) conjugate or with streptavidin-Cy3 (red) conjugate to stain for the biotinylated ricinB.